Chicken anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, rabbit IgG/serum

Product no: AS10 1107

AS10 1107 | Clonality: Polyclonal  |  Host: Chicken   |  Reactivity: Rat IgG (H&L)

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Delivery:  3-6 business days
  • Product Info
  • Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY.
    Format: Lyophilized
    Quantity: 0.5 mg
    Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative.

  • Background
  • Background: Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
  • Protocols
  • Antibody protocols
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