Anti-V-ATPase, c | Vacuolar H+-ATPase, subunit c (16 kDa)

Product no: AS09 468

AS09 468  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, M. crystallinum, N. tabacum

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c UniProt: Q6IDA4, TAIR: AT2G16510

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    16 | 16 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum
    Predicted reactivity:

    dictos including: Cucumis sativus, Gossypium mexicanum, Manihot esculenta, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa


    Species of your interest not listed? Contact us
    Not reactive in: Algae
  • Application Examples
  • Application example

    western blot using anti-V ATPase subunit c antibodies
    Following samples were analyzed: MW markers (1), Arabidopsis thaliana tonoplast (2), Arabidopsis thaliana plasma membrane (3), Arabidopsis thaliana microsomes (4), Arabidopsis thaliana total protein (5)Mesembryanthemum crystallinum tonoplast (6), Mesembryanthemum crystallinum plasma membrane (7)Mesembryanthemum crystallinum microsomes (8), Nicotiana tabacum microsomes (9), Brassica napus microsomes (10). 15 µg of the indicated protein, extracted according to Vera-Estrella et al. (2012) was separated on 12% SDS-PAGE and blotted 1.15h to PVDF using tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 O/N at RT with agitation. The antibody solution was decanted and the blot was washed 3X for 15 min min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602 ) diluted to 1:50 000 in for 2h at RT with agitation. The blot was washed as above and developed using chemiluminescent substrated and recorder using the LiCOR c-DIGIT personal imager.

    Courtesy Dr. Bronwyn Barkla. UNAM, Mexico
  • Additional Information
  • Additional information:
    Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min.
    Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel
  • Background
  • Background:

    V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300

  • Product Citations
  • Selected references: Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.
    Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela




    Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

218 €
Add to cart
Info
img missing
AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €
Close