To directly conjugated a primary antibody to an enzyme, like ALP or HRP, or a fluorescent dye, like DyLight®, the antibody must be of the highest purity; purified against the specific antigen. Q: What are the benefits of using directly conjugated primary antibodies? A: The time necessary to complete all steps of a Western blot or immunolocalization protocol, is shortened considerably, as there is no need for secondary antibody incubation and subsequent washes, before the final reaction can be visualized. Potential cross-reactions, which may occur when using secondary antibodies, are also eliminated. Possible sample loss due to washing steps is also reduced. Until end of 2024, all directly conjugated Agrisera primary antibodies are discounted by 10%, when applying the discount code Primconj10. Check out the complete Agrisera collection of directly conjugated primary antibodies here. | Using directly conjugated primary antibodies, is most beneficial for protein targets of high and moderate abundance in the analyzed tissue. |
Latest
Agrisera supports 9th Plant Biochemistry Symposium: Plant Metabolites and Signaling2024-05-17 Track changes in LHC proteins using most published anti-LHC antibodies
2024-05-12 Agrisera supports BioLock student conference at the University of Wrocław
2024-05-10 Secondary Antibody Guide
2024-05-06 Shorten the time of Western blot or IL by using directly conjugated primary antibodies
2024-05-03 20 % off for antibodies to proteins involved in electron transfer in photosynthesis
2024-04-26 Get new tips and tricks for Western blot during Agrisera workshop on 16th of May at 11 am CET
2024-04-25 Detect and quantify Rubisco using antibodies with 20 years of publication record
2024-04-19 Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?
2024-04-17 Study Photosystem I with Agrisera comprehensive antibody set
2024-04-14
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